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Peer reviewedOpen accessAIDS

Siglec-1-targeted nanobodies restrict HIV-1 transmission and infection of dendritic cells

Journal of Virology·

Shirley Man, Alsya J. Affandi, Hendrik J. Brink, John L. van Hamme, Veronique A. L. Konijn, Joeke G. C. Stolwijk, Joke M. M. den Haan, Neeltje A. Kootstra, Teunis B. H. Geijtenbeek

DOI
10.1128/jvi.00128-26
PMID
42523118
PMCID
OpenAlex
W7171721589
Study type
Journal article
Publisher
American Society for Microbiology
Article type
journal-article
Integrity
current

Why this research matters now

The data indicate that interfering with host attachment molecules could theoretically disrupt initial viral dissemination pathways, supporting continued exploration of such mechanisms.

01

Structured evidence summary

Research question

Does neutralizing the Siglec-1 receptor with engineered nanobodies prevent dendritic cells from capturing and transferring HIV-1?

Study design

An in vitro laboratory experiment assessing the binding interference and cellular impact of single-domain antibody fragments across modified cell lines and dendritic cultures.

Population and setting

The investigation relies exclusively on cultured cell systems rather than human subjects or in vivo models.

Main findings

Targeted nanobodies successfully inhibited viral attachment and subsequent infection in receptor-overexpressing models. Application to dendritic cells stopped both replication-dependent and independent viral transfer while avoiding unintended immune stimulation.

Public-health relevance

The data indicate that interfering with host attachment molecules could theoretically disrupt initial viral dissemination pathways, supporting continued exploration of such mechanisms.

Important limitations

The evaluation relies solely on in vitro cell culture systems, meaning physiological complexity, systemic pharmacokinetics, and human safety remain unaddressed.

GIDS interpretation

This manuscript documents preclinical validation of a receptor-specific inhibitory agent, offering background reference for scholarly assessments of host-targeted antiviral development.

02

Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

03

Evidence relationships

This article has 8 auditable classifier relationships to diseases, places, topics, and study design.

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