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Peer reviewedOpen accessMonkeypoxSmallpox

APOBEC3-driven attenuation of the 2022 global outbreak monkeypox virus relative to its clade IIb ancestor

Nature Communications·

Rebecca P. Sumner, Lucy Eke, Bruno Hernáez, Alasdair J. M. Hood, Telma Sancheira Freitas, Hannah Ashby, Ailish Ellis, Marine Petit, Sian Lant, Isobel Stokes, Preetam Parija, Isabel Alonso, Francisco J. Alvarez-de Miranda, Alazne R. Unanue, Kevin Maringer, Isabelle Dietrich, Bryan Charleston, Geoffrey L. Smith, David O. Ulaeto, Antonio Alcamí, Carlos Maluquer de Motes

DOI
10.1038/s41467-026-76580-7
PMID
PMCID
OpenAlex
W7202172697
Study type
Journal article
Publisher
Springer Science and Business Media LLC
Article type
journal-article
Integrity
current

Why this research matters now

The article is relevant to monkeypox outbreak investigation and transmission dynamics because it addresses phenotypic differences in a virus lineage associated with the 2022 global epidemic. Its focus on zoonotic MPXV and orthopoxvirus transmission gives it One Health relevance.

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Structured evidence summary

Research question

The article examines how the 2022 global outbreak monkeypox virus lineage B.1 differs phenotypically from a closely related earlier clade IIb lineage A.1 virus, with attention to APOBEC3-associated mutations.

Study design

The abstract describes a comparative laboratory study using viral replication and spread assays, innate immune response analyses, mutation mapping, and a mouse model of monkeypox virus infection.

Population and setting

The setting includes clade IIb monkeypox virus lineages associated with West Africa and the 2022 global outbreak, plus experimental cell culture and mouse infection model systems. No human participant population is described in the abstract.

Main findings

The abstract reports that lineage B.1 and A.1 showed similar replication in cell culture, while B.1 had impaired longer-distance spread linked to an APOBEC3-associated change in OPG057. It also reports altered innate immune control linked to an OPG047 change and lower virulence in a mouse model compared with A.1.

Public-health relevance

The article is relevant to monkeypox outbreak investigation and transmission dynamics because it addresses phenotypic differences in a virus lineage associated with the 2022 global epidemic. Its focus on zoonotic MPXV and orthopoxvirus transmission gives it One Health relevance.

Important limitations

The abstract indicates reliance on cell culture experiments and a mouse infection model, so direct interpretation for human disease severity or transmission is limited from the supplied summary alone. This evidence summary is limited to the supplied single-article abstract and metadata and requires the original paper for decision-grade interpretation.

GIDS interpretation

The classifier links make this article discoverable under monkeypox, smallpox, One Health, outbreak investigation, and transmission dynamics. These links provide indexing context only and do not establish any connection to a live surveillance signal.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

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Evidence relationships

This article has 8 auditable classifier relationships to diseases, places, topics, and study design.

about diseaseaddresses topicaddresses topicaddresses topichas pathogen typestudied population settingstudies pathogenuses study design