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Peer reviewedOpen accessInfluenzaH5N1

Dual-route H5N1 vaccination induces systemic and mucosal immunity in murine and bovine models

npj Vaccines·

Joshua Wiggins, Adthakorn Madapong, Eric A. Weaver

DOI
10.1038/s41541-026-01460-6
PMID
42014782
PMCID
PMC13284322
OpenAlex
W4414402707
Study type
Genomic study
Publisher
Springer Science and Business Media LLC
Article type
journal-article
Integrity
current

Why this research matters now

The investigation addresses agricultural economic concerns and zoonotic transmission potential by evaluating livestock-targeted immunization strategies against expanding avian influenza lineages.

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Structured evidence summary

Research question

How effectively does a dual-administration H5N1 vaccine regimen generate protective immune responses in murine and bovine subjects?

Study design

Controlled preclinical experiment utilizing prime-boost immunization followed by pathogen challenge in laboratory mice and calves.

Population and setting

Murine and bovine animal models maintained under controlled experimental conditions.

Main findings

The immunization protocol stimulated robust humoral and cellular responses across both species. Challenged mice demonstrated complete survival against lethal exposure to divergent H5N1 isolates, including a recently identified bovine strain. Immune profiles remained comparable between animals, indicating potential cross-species relevance.

Public-health relevance

The investigation addresses agricultural economic concerns and zoonotic transmission potential by evaluating livestock-targeted immunization strategies against expanding avian influenza lineages.

Important limitations

Outcomes are confined to controlled animal experiments, preventing direct assessment of efficacy in natural herd settings or human populations.

GIDS interpretation

This publication contributes to the broader literature on avian influenza genomics and veterinary vaccine development. It provides preclinical context for immunogen selection and delivery methods without reflecting real-time outbreak monitoring or active surveillance activity.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

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Evidence relationships

This article has 13 auditable classifier relationships to diseases, places, topics, and study design.

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