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Peer reviewedOpen accessGastroenteritis

Norovirus inter-species transmission in an ex vivo model is dependent on virus internalization capacity

Emerging Microbes & Infections·

Nele Villabruna, Myra Hosmillo, Ian Goodfellow, Niklas Arnberg, Juan Bárcena, Silke Rautenschlein, Benjamin Heidrich, Andreas Beineke, Gisa Gerold

DOI
10.1080/22221751.2026.2713851
PMID
42546135
PMCID
OpenAlex
W7172272484
Study type
Journal article
Publisher
Informa UK Limited
Article type
journal-article
Integrity
current

Why this research matters now

Clarifying tissue compatibility between human and domestic animal viruses aids One Health frameworks in mapping potential zoonotic interfaces and guiding cross-sectoral monitoring priorities.

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Structured evidence summary

Research question

The investigation evaluates whether noroviruses can breach species boundaries by assessing viral attachment and cellular entry across intestinal tissues from multiple animal hosts.

Study design

Authors conducted an ex vivo experiment utilizing precision-cut intestinal slices obtained from swine, canines, and poultry.

Population and setting

The research relies exclusively on isolated tissue models rather than living clinical cohorts or field-based animal populations.

Main findings

Human norovirus particles effectively bound to and entered pig and dog intestinal tissue at levels matching native animal strains, with successful replication observed in porcine samples. Chicken tissue exhibited zero viral interaction, while animal noroviruses showed minimal cellular penetration in human tissue. These patterns suggest that efficient viral uptake acts as a biological filter, making human-to-domestic animal spread more feasible than the opposite direction.

Public-health relevance

Clarifying tissue compatibility between human and domestic animal viruses aids One Health frameworks in mapping potential zoonotic interfaces and guiding cross-sectoral monitoring priorities.

Important limitations

Utilizing isolated intestinal slices prevents evaluation of systemic immune defenses and whole-organism physiological variables that would shape actual infection trajectories.

GIDS interpretation

The manuscript supplies mechanistic data on host-range constraints that could help researchers prioritize viral candidates for future genomic screening efforts within integrated health observation networks.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

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Evidence relationships

This article has 6 auditable classifier relationships to diseases, places, topics, and study design.

about diseaseaddresses topicaddresses topichas pathogen typestudied population settinguses study design