The pair of fatty acid degradation regulator (FadR)-characterized pseudo-palindromic motifs spacing with 11 bp contribute unequally to the DNA binding ability of the HTH-type transcriptional regulator Rv0494 from Mycobacterium tuberculosis H37Rv.
International journal of biological macromolecules·
- DOI
- 10.1016/j.ijbiomac.2026.154065
- PMID
- 42603683
- PMCID
- —
- OpenAlex
- W7203536179
- Study type
- Journal article
- Publisher
- Publisher unavailable
- Article type
- journal-article
- Integrity
- current
Why this research matters now
Understanding how Rv0494 regulates persistence, starvation response, and very-long-chain fatty acid synthesis in Mycobacterium tuberculosis may inform insights into bacterial adaptation and survival mechanisms relevant to tuberculosis pathogenesis.
Structured evidence summary
Research question
The study investigated how DNA sequence features, particularly a pair of FadR-characterized pseudo-palindromic motifs separated by 11 base pairs, influence the DNA binding ability of the HTH-type transcriptional regulator Rv0494 in Mycobacterium tuberculosis H37Rv.
Study design
The authors used structural biology and biochemical approaches to characterize the DNA sequence dependence and binding behavior of Rv0494.
Population and setting
The study examined Mycobacterium tuberculosis H37Rv, the pathogen responsible for tuberculosis, with a focus on the transcriptional regulator Rv0494 and its interaction with upstream sequences of carbon metabolism-related genes.
Main findings
Rv0494 binds DNA using conserved FadR mechanisms, including key residues and functional homodimers, but the two FadR-characterized pseudo-palindromic motifs contribute unequally to binding. An additional palindromic sequence with a 5 bp spacer, formed by one pseudo-palindromic motif and a complementary sequence, appears to enhance binding activity asymmetrically. This unequal motif dependence was validated in upstream regions of carbon metabolism genes such as icl1, though DNA binding does not always result in transcriptional repression.
Public-health relevance
Understanding how Rv0494 regulates persistence, starvation response, and very-long-chain fatty acid synthesis in Mycobacterium tuberculosis may inform insights into bacterial adaptation and survival mechanisms relevant to tuberculosis pathogenesis.
Important limitations
This summary relies on a single-article abstract and metadata. Full interpretation of the structural biology methods, binding assay conditions, validation scope, and the relationship between DNA binding and transcriptional outcomes requires access to the complete published manuscript.
GIDS interpretation
The article was classified under Tuberculosis and Climate and environment topics, likely reflecting the focus on Mycobacterium tuberculosis biology and bacterial response to environmental stressors such as carbon source scarcity. This classification aids discoverability for researchers studying pathogen adaptation and metabolic regulation.
Related GIDS surveillance
Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.
Evidence relationships
This article has 4 auditable classifier relationships to diseases, places, topics, and study design.