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Peer reviewedOpen accessSARSCOVID-19

Preclinical evaluation of a plant-derived SARS-CoV-2 subunit vaccine: Protective efficacy, immunogenicity, safety, and toxicity

Vaccine·

Balamurugan Shanmugaraj, Narach Khorattanakulchai, Chalisa Panapitakkul, Ashwini Malla, Rawiwan Im-erbsin, Manutsanun Inthawong, Piyanate Sunyakumthorn, Taweewun Hunsawong, Chonticha Klungthong, Matthew C. Reed, Taratorn Kemthong, Nutchanat Suttisan, Suchinda Malaivijitnond, Passaraporn Srimangkornkaew, Aekkarin Klinkhamhom, Suwimon Manopwisedjaroen, Arunee Thitithanyanont, Suthira Taychakhoonavudh, Waranyoo Phoolcharoen

DOI
10.1016/j.vaccine.2022.05.087
PMID
35697573
PMCID
PMC9167921
OpenAlex
W4282031643
Study type
Journal article
Publisher
Elsevier BV
Article type
journal-article
Integrity
current

Why this research matters now

The study presents preclinical evidence for a plant-based vaccine platform that demonstrated protective immunity and acceptable safety in animal models, suggesting potential applicability for COVID-19 vaccine development.

01

Structured evidence summary

Research question

This study evaluated the protective efficacy, safety, and toxicity of a plant-derived protein subunit vaccine (Baiya SARS-CoV-2 Vax 1) in preclinical models.

Study design

A preclinical study evaluated a two-dose vaccine regimen (administered on Days 0 and 21) across K18-hACE2 mice, cynomolgus monkeys, and Wistar rats, assessing protective efficacy, safety pharmacology, and toxicology.

Population and setting

Experimental animal models including K18-hACE2 mice (for challenge and protective efficacy assessment), cynomolgus monkeys (for safety pharmacology), and Wistar rats (for toxicology evaluation) were used.

Main findings

In K18-hACE2 mice, two vaccine doses reduced SARS-CoV-2 viral loads in lungs and brains and protected against viral challenge. In monkeys, safety pharmacology testing of the central nervous, cardiovascular, and respiratory systems revealed no safety concerns. In rats, toxicology assessment showed no vaccine-related pathological changes, with healthy outcomes and clinical tolerance even at the highest tested concentration.

Public-health relevance

The study presents preclinical evidence for a plant-based vaccine platform that demonstrated protective immunity and acceptable safety in animal models, suggesting potential applicability for COVID-19 vaccine development.

Important limitations

This summary is limited to the supplied single-article abstract and metadata; the original full text is required for decision-grade interpretation. The study reports preclinical animal data only, without human clinical trial evidence, and specific details on population demographics or study setting are not available in the abstract.

GIDS interpretation

This article describes preclinical vaccine research and is catalogued under vaccination, SARS, and COVID-19 topics. No surveillance signal connection is established or inferred from this evidence alone.

02

Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

03

Evidence relationships

This article has 10 auditable classifier relationships to diseases, places, topics, and study design.

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