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Peer reviewedOpen accessLeishmaniasis

Recombinant vaccine plus anti-parasitic agent in an immunotherapeutic schedules induce partial regression of tegumentary leishmaniasis in a murine model

Vaccine·

Camila S. Freitas, Daniela P. Lage, Breno L. Pimenta, Karolina O.M. Falcão, Gabriel J.L. Moreira, Isabela A.G. Pereira, Raquel S.B. Câmara, Stéphanie L.P. Simão, Ana L. Silva, João A. Oliveira-da-Silva, Dóris M. Abrão, Alexsandro S. Galdino, Miguel A. Chávez-Fumagalli, Bruno M. Roatt, Myron Christodoulides, Eduardo A.F. Coelho

DOI
10.1016/j.vaccine.2026.128990
PMID
42546635
PMCID
OpenAlex
W7172300930
Study type
Journal article
Publisher
Elsevier BV
Article type
journal-article
Integrity
current

Why this research matters now

Current management options for tegumentary leishmaniasis face constraints regarding efficacy and adverse effects. This laboratory-based analysis highlights a potentially safer and more effective combinatorial approach that warrants subsequent developmental stages.

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Structured evidence summary

Research question

The investigation assesses whether a combined regimen of a recombinant protein, an adjuvant, and an antiparasitic medication can effectively manage experimental leishmaniasis in a laboratory animal model.

Study design

This represents a controlled preclinical trial utilizing a murine infection model. Animals received targeted therapies and underwent parasitological, immunological, and biochemical assessments at one-month intervals following intervention.

Population and setting

The research utilized BALB/c mice experimentally challenged with Leishmania amazonensis. No human subjects or field-based clinical environments were involved in the protocol.

Main findings

The three-drug regimen demonstrated superior therapeutic outcomes compared to all other tested groups. Treated subjects displayed heightened Th1-mediated immune activation alongside marked reductions in tissue parasite counts. Standard hematological and organ function indicators remained within normal ranges throughout the observation period.

Public-health relevance

Current management options for tegumentary leishmaniasis face constraints regarding efficacy and adverse effects. This laboratory-based analysis highlights a potentially safer and more effective combinatorial approach that warrants subsequent developmental stages.

Important limitations

The experimental framework relies exclusively on a single inbred mouse strain and a defined parasite isolate. Consequently, translational applicability to human populations remains unverified within this report.

GIDS interpretation

Indexed under treatment and vaccination categories, this publication offers mechanistic data on a novel immunotherapeutic construct. Researchers monitoring combinatorial interventions for neglected parasitic diseases may find the reported immune profiling and safety metrics useful for contextual mapping.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

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Evidence relationships

This article has 8 auditable classifier relationships to diseases, places, topics, and study design.

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