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Peer reviewedOpen accessCOVID-19SARS

Identification of cross reactive T cell responses in adenovirus based COVID 19 vaccines

npj Vaccines·

Joshua Gardner, Simon Timothy Abrams, Cheng-Hock Toh, Alan L. Parker, Charlotte Lovatt, Phillip L. R. Nicolson, Steve P. Watson, Sophie Grice, Luisa Hering, Munir Pirmohamed, Dean J. Naisbitt

DOI
10.1038/s41541-024-00895-z
PMID
PMCID
OpenAlex
W4399401281
Study type
Journal article
Publisher
Springer Science and Business Media LLC
Article type
journal-article
Integrity
current

Why this research matters now

The findings relate to understanding T-cell responses to adenovirus-vectored COVID-19 vaccines and potential cross-reactivity with prevalent human adenoviruses, with implications for vaccine platform selection and safety profiling.

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Structured evidence summary

Research question

The study investigated patterns of T-cell activation in response to adenovirus-vectored COVID-19 vaccines, focusing on pre-pandemic samples from vaccine-naïve donors and vaccinated controls.

Study design

In vitro immunology study using PBMCs from pre-pandemic ChAdOx1 nCoV-19 naïve healthy donors and vaccinated controls. T-cell proliferation was assessed via lymphocyte transformation test, with cytokine analysis performed through intracellular cytokine staining, ELISpot assay, and LEGENDplex immunoassays.

Population and setting

Healthy donors recruited prior to the SARS-CoV-2 pandemic (vaccine-naïve) and controls who received ChAdOx1 nCoV-19 or Pfizer vaccination.

Main findings

Widespread lymphocyte stimulation occurred after exposure to ChAdOx1 nCoV-19 (95%), ChAdOx-spike (90%), and Ad26.COV2.S vaccine in pre-pandemic vaccine-naïve donors, but not with BNT162b2 vaccine. CD4+ CD45RO+ memory T-cells were activated by ChAdOx1 nCoV-19 in vaccine-naïve donors. Exposure was associated with release of proinflammatory and cytotoxic molecules including IFN-γ, IL-6, perforin, granzyme B, and FasL.

Public-health relevance

The findings relate to understanding T-cell responses to adenovirus-vectored COVID-19 vaccines and potential cross-reactivity with prevalent human adenoviruses, with implications for vaccine platform selection and safety profiling.

Important limitations

This summary relies on the supplied single-article abstract and metadata. The original paper is required for decision-grade interpretation, including detailed methods, statistical analyses, sample size justification, and discussion of confounders.

GIDS interpretation

The article is indexed under COVID-19, SARS, vaccination, treatment, and outbreak investigation topics. The work examines immunological mechanisms relevant to adenovirus-vectored vaccine responses. Cross-reactive T-cell responses identified in pre-pandemic samples may inform understanding of baseline immunity patterns.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

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Evidence relationships

This article has 11 auditable classifier relationships to diseases, places, topics, and study design.

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