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Peer reviewedOpen accessInfluenzaH5N1

Receptor profiling and growth assessment of influenza A virus in porcine mammary and non-mammary tissues and derived cells

Journal of Virology·

Ulises Barron-Castillo, Nathalie Berube, Cynthia L. Swan, M. Afzal Javed, Lauren Aubrey, Jill Trann, Makenzie Gidych, Sauhard Shrivastava, Kaushal Baid, Arinjay Banerjee, Yan Zhou

DOI
10.1128/jvi.00615-26
PMID
42405777
PMCID
OpenAlex
W7167480187
Study type
Journal article
Publisher
American Society for Microbiology
Article type
journal-article
Integrity
current

Why this research matters now

Identifying alternative replication niches in livestock anatomy aids in evaluating cross-species transmission risks and monitoring viral adaptation pathways in agricultural environments.

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Structured evidence summary

Research question

The investigation seeks to determine whether porcine mammary tissue supports influenza A virus entry and replication compared to respiratory tract tissue.

Study design

This laboratory experiment characterized viral receptor distribution, measured binding affinities, and quantified viral growth across isolated and immortalized epithelial cells from swine mammary and respiratory origins.

Population and setting

Research utilized porcine mammary and respiratory tract samples alongside corresponding primary and immortalized cell cultures.

Main findings

Porcine mammary tissue expresses both SA-α2,3 and SA-α2,6 receptors, enabling variable viral attachment. Bovine H5N1 isolates demonstrate efficient replication in both mammary and respiratory cell lines, while other avian, swine, and human strains exhibit moderate growth in mammary epithelium but robust proliferation in respiratory counterparts. These results indicate that swine mammary tissue functions as a viable environment for viral multiplication and potential genetic reassortment.

Public-health relevance

Identifying alternative replication niches in livestock anatomy aids in evaluating cross-species transmission risks and monitoring viral adaptation pathways in agricultural environments.

Important limitations

The evaluation depends entirely on the provided abstract and bibliographic metadata, requiring access to the complete manuscript for comprehensive methodological verification and decision-grade interpretation. No explicit constraints were detailed within the supplied source material.

GIDS interpretation

This report contributes baseline virological context regarding host tissue compatibility that may guide future investigative priorities within the One Health domain, without implying direct alignment with ongoing epidemiological tracking initiatives.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

03

Evidence relationships

This article has 6 auditable classifier relationships to diseases, places, topics, and study design.

about diseaseaddresses topichas pathogen typestudied population settingstudies pathogenuses study design