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Peer reviewedOpen accessInfluenzaH5N1

A bivalent inactivated influenza vaccine incorporating epitope-optimized surface proteins confers cross-protective immunity against H9N2 influenza virus

Emerging Microbes & Infections·

Mengchan Hao, Yiwei Guan, Meng Xu, Wenxue Yang, Yanhai Wang, Yuan Zhang, Jianjun Chen

DOI
10.1080/22221751.2026.2713325
PMID
42546126
PMCID
OpenAlex
W7172254248
Study type
Journal article
Publisher
Informa UK Limited
Article type
journal-article
Integrity
current

Why this research matters now

The work addresses agricultural and zoonotic risk management by testing a sequence-driven vaccine strategy intended to sustain efficacy despite continuous viral mutation.

01

Structured evidence summary

Research question

The investigation examines whether a computationally refined bivalent inactivated formulation can generate broader immune defense against evolving H9N2 avian influenza strains compared to established reference candidates.

Study design

Researchers performed a preclinical murine trial comparing the novel chimeric vaccine against a standard candidate virus and evaluating survival outcomes after exposure to a mismatched viral isolate.

Population and setting

Laboratory mice served as the exclusive biological model for all immunogenicity assessments and pathogen challenge experiments.

Main findings

Animals administered the optimized dual-component preparation showed heightened cross-reactive antibody generation and T-cell activation relative to the comparator strain. Vaccinated subjects also maintained survival following lethal exposure to a genetically divergent H9N2 isolate and displayed diminished respiratory viral replication.

Public-health relevance

The work addresses agricultural and zoonotic risk management by testing a sequence-driven vaccine strategy intended to sustain efficacy despite continuous viral mutation.

Important limitations

The analysis depends entirely on murine models, which prevents direct translation to human immunological responses or practical application in commercial poultry operations without additional validation.

GIDS interpretation

This peer-reviewed publication documents preclinical progress in algorithm-guided influenza vaccine engineering, offering context for ongoing academic efforts to manage antigenically variable avian strains under a One Health perspective.

02

Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

03

Evidence relationships

This article has 8 auditable classifier relationships to diseases, places, topics, and study design.

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