Development and validation of indirect ELISAs based on multiepitope recombinant outer membrane protein fusion antigens for caprine brucellosis diagnosis
BMC Veterinary Research·
- DOI
- 10.1186/s12917-026-05892-z
- PMID
- —
- PMCID
- —
- OpenAlex
- W7210294684
- Study type
- Journal article
- Publisher
- Springer Science and Business Media LLC
- Article type
- journal-article
- Integrity
- current
Why this research matters now
The authors suggest these recombinant antigen-based ELISAs could serve as cost-effective tools for caprine brucellosis diagnosis and surveillance, with relevance given that Brucella melitensis in goats is the most virulent species for humans.
Structured evidence summary
Research question
The study investigates whether indirect ELISAs based on multiepitope recombinant outer membrane protein fusion antigens can provide accurate serodiagnosis of caprine brucellosis.
Study design
The work is a laboratory-based diagnostic development and validation study evaluating three recombinant fusion antigens as indirect ELISAs against reference serological tests using a panel of 400 goat serum samples.
Population and setting
The evaluation used 400 goat serum samples and compared assay performance against combined Rose Bengal and microtiter plate agglutination tests and a commercial ELISA, in a setting framed by caprine brucellosis as a livestock and zoonotic concern.
Main findings
Two fusion-protein ELISAs showed robust performance: OMP31/28/25 reached 91.34% sensitivity and 84.62% specificity versus RBT+MPAT, and 91.39% sensitivity and 91.97% specificity versus the commercial ELISA. OMP28/25 reached 95.28%/82.78% versus RBT+MPAT and 94.7%/89.96% versus the commercial ELISA. Cohen's kappa values of 0.71–0.83 indicated good-to-very good agreement. The single OMP31 ELISA was reported as having inadequate diagnostic performance.
Public-health relevance
The authors suggest these recombinant antigen-based ELISAs could serve as cost-effective tools for caprine brucellosis diagnosis and surveillance, with relevance given that Brucella melitensis in goats is the most virulent species for humans.
Important limitations
The authors note that broader field validation is needed. This summary is limited to the supplied single-article abstract/metadata and requires the original paper for decision-grade interpretation.
GIDS interpretation
Within the GIDS literature evidence framework, this article is discoverable as a diagnostic-development record relevant to brucellosis serology in goats and is tagged for diagnostics, One Health, and surveillance; no live surveillance signal is confirmed by this single source.
Related GIDS surveillance
Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.
Evidence relationships
This article has 9 auditable classifier relationships to diseases, places, topics, and study design.