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Peer reviewedOpen accessGastroenteritisInfluenza

Mechanisms of GII.4 Norovirus Persistence in Human Populations

PLoS Medicine·

Lisa C Lindesmith, Eric F Donaldson, Anna D LoBue, Jennifer L Cannon, Du-Ping Zheng, Jan Vinje, Ralph S Baric

DOI
10.1371/journal.pmed.0050031
PMID
18271619
PMCID
PMC2235898
OpenAlex
W2113585031
Study type
Genomic study
Publisher
Public Library of Science (PLoS)
Article type
journal-article
Integrity
current

Why this research matters now

Findings are positioned as a rationale for vaccine development against noroviruses and for ongoing epidemiologic surveillance with vaccine reformulation, analogous to influenza control strategies.

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Structured evidence summary

Research question

The study investigates the molecular mechanisms underlying the epidemiological persistence, strain replacement, and host-susceptibility patterns of GII.4 noroviruses in human populations.

Study design

A genomic and laboratory-based molecular analysis combining phylogenetic evaluation of GII.4 capsid (ORF2) sequences with expression of representative virus-like particles (VLPs), salivary and carbohydrate-binding assays, and antigenic relatedness measurements using murine and human sera.

Population and setting

Human populations in community, military, cruise-ship, hospital, and assisted-living outbreak settings, examined via molecular sequence data and serum/saliva samples from humans and mice used in binding and serologic assays.

Main findings

GII.4 capsid sequences showed an epochal evolutionary pattern over roughly 20 years, with five sequential clusters emerging in linear temporal succession. VLP binding assays indicated that HBGA carbohydrate-recognition profiles broadened over time to include ligands regulated by both FUT2 and FUT3 pathways. Strain-specific serologic and carbohydrate-binding blockade responses were observed, consistent with antigenic drift in surface-exposed capsid residues near the ligand-binding region.

Public-health relevance

Findings are positioned as a rationale for vaccine development against noroviruses and for ongoing epidemiologic surveillance with vaccine reformulation, analogous to influenza control strategies.

Important limitations

No explicit limitations are stated in the supplied abstract. This summary is limited to the supplied single-article abstract and bibliographic metadata; the original paper is required for decision-grade interpretation, including assessment of assay validation, sample sizes, and phylogenetic methodology.

GIDS interpretation

This article is indexed under genomic epidemiology and outbreak investigation themes and may aid discoverability of mechanistic literature on GII.4 norovirus evolution; it does not, on its own, constitute a live surveillance signal.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

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Evidence relationships

This article has 7 auditable classifier relationships to diseases, places, topics, and study design.

about diseaseaddresses topicaddresses topicaddresses topichas pathogen typestudied population settinguses study design