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Peer reviewedOpen accessRift Valley fever

Aerosol Exposure to Rift Valley Fever Virus Causes Earlier and More Severe Neuropathology in the Murine Model, which Has Important Implications for Therapeutic Development

PLoS Neglected Tropical Diseases·

Christopher Reed, Kenny Lin, Catherine Wilhelmsen, Brian Friedrich, Aysegul Nalca, Ashley Keeney, Ginger Donnelly, Joshua Shamblin, Lisa E. Hensley, Gene Olinger, Darci R. Smith

DOI
10.1371/journal.pntd.0002156
PMID
23593523
PMCID
PMC3617210
OpenAlex
W2115756207
Study type
Journal article
Publisher
Public Library of Science (PLoS)
Article type
journal-article
Integrity
current

Why this research matters now

The authors emphasize that aerosol-transmitted RVFV infection is a relevant but under-characterized exposure route and that the route influences pathogenesis and the apparent effectiveness of at least one candidate therapeutic, underscoring the need for additional antivirals and route-appropriate evaluation of vaccines and therapeutics.

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Structured evidence summary

Research question

The study examined how aerosol versus subcutaneous exposure to Rift Valley fever virus alters disease pathogenesis in mice, and whether the nucleoside analog ribavirin given prophylactically differs in effectiveness between these routes.

Study design

A controlled murine comparison of aerosol versus subcutaneous RVFV exposure, evaluating survival, clinical observations, blood chemistry, hematology, immunohistochemistry, and tissue virus titers, with a prophylactic ribavirin treatment arm applied across both routes.

Population and setting

A laboratory-based murine (mouse) experimental model; no human participants or geographic setting are described.

Main findings

Survival, clinical chemistry, and hematology were not significantly affected by exposure route, and acute hepatitis developed in both groups. Neuropathology appeared earlier and was more severe following aerosol exposure than after subcutaneous exposure. Ribavirin provided partial protection to subcutaneous-exposed mice but did not protect aerosol-exposed mice, with early aggressive brain invasion implicated in treatment failure.

Public-health relevance

The authors emphasize that aerosol-transmitted RVFV infection is a relevant but under-characterized exposure route and that the route influences pathogenesis and the apparent effectiveness of at least one candidate therapeutic, underscoring the need for additional antivirals and route-appropriate evaluation of vaccines and therapeutics.

Important limitations

The abstract does not enumerate explicit limitations of the study. This summary is limited to the supplied single-article abstract and bibliographic metadata; the original paper is required for decision-grade interpretation, including assessment of sample sizes, statistical methods, and any author-reported constraints.

GIDS interpretation

The article is discoverable under the Rift Valley fever disease tag and is classified to transmission dynamics, treatment, and vaccination topics, suggesting it may serve as context for signal interpretation involving exposure-route effects on RVFV pathogenesis or therapeutic response. No connection to a live surveillance signal is made or implied.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

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Evidence relationships

This article has 9 auditable classifier relationships to diseases, places, topics, and study design.

about diseaseaddresses topicaddresses topicaddresses topicevaluates interventionevaluates interventionhas pathogen typestudied population settinguses study design