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Peer reviewedOpen accessBrucellosis

Brucella abortus and public health risk: Prevalence in milk sold at open markets in Cameroon

PLOS Neglected Tropical Diseases·

Pierre Gontao, Christopher G. Laine, Gaelle Kamdjo Guela, Charles Olivier Gomsu Dada, Hortense Abiba, Charles Félix Bilong Bilong, Wilfred Mbacham, Daniel G. Garcia-Gonzalez, Sonia Vection, John D. Gillece, Jeffrey T. Foster, Abel Wade, Angela M. Arenas-Gamboa

DOI
10.1371/journal.pntd.0014051
PMID
PMCID
OpenAlex
W7140141832
Study type
Journal article
Publisher
Public Library of Science (PLoS)
Article type
journal-article
Integrity
current

Why this research matters now

The detection of B. abortus in milk sold at open markets, combined with widespread risky practices among merchants (low boiling rates and milk pooling), indicates a pathway for human brucellosis exposure beyond traditional farm and abattoir settings.

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Structured evidence summary

Research question

This study investigated the presence of Brucella spp. in milk sold at open-air markets across three regions of Cameroon (Far North, North, and West).

Study design

A cross-sectional survey was conducted using 1,140 milk samples collected from 380 merchants in each of three regions. Samples underwent iELISA, culture, real-time PCR, and next-generation sequencing (NGS), along with KAP (knowledge, attitudes, and practices) surveys of merchants.

Population and setting

Milk merchants at open-air markets in the Far North, North, and West regions of Cameroon, where milk is sold for human consumption without regulation.

Main findings

B. abortus was detected in milk sold for human consumption, with culture positivity at 1.3% countrywide and real-time PCR positivity at 4.0%. Anti-Brucella antibodies were found in 21% of samples, with regional variation (38.4% Far North, 15.5% North, 8.9% West). NGS confirmed bacterial isolates from milk were epidemiologically linked to those from animals. KAP surveys revealed only 26.4% of merchants boil milk, while 91.2% mix milk from multiple cattle. All merchants selling culture-positive milk engaged in risky behaviors, potentially exposing approximately 720 people to contaminated milk weekly.

Public-health relevance

The detection of B. abortus in milk sold at open markets, combined with widespread risky practices among merchants (low boiling rates and milk pooling), indicates a pathway for human brucellosis exposure beyond traditional farm and abattoir settings.

Important limitations

This summary is limited to the supplied single-article abstract/metadata and requires the original paper for decision-grade interpretation. The study reflects a single-point cross-sectional survey in three regions of Cameroon and does not provide data on milk consumer habits or human infection prevalence.

GIDS interpretation

This article provides context on brucellosis surveillance and food safety monitoring in Cameroon. No surveillance signal is reported or inferable from the supplied abstract.

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Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

03

Evidence relationships

This article has 7 auditable classifier relationships to diseases, places, topics, and study design.

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