Value of routine whole genome sequencing for Mycobacterium tuberculosis drug resistance detection
International Journal of Infectious Diseases·
- DOI
- 10.1016/j.ijid.2021.03.033
- PMID
- 33753222
- PMCID
- —
- OpenAlex
- W3136232976
- Study type
- Genomic study
- Publisher
- Elsevier BV
- Article type
- journal-article
- Integrity
- current
Why this research matters now
The abstract frames routine sequencing as relevant to tuberculosis drug resistance detection and management in low-incidence settings. The article is indexed to tuberculosis, antimicrobial resistance, and genomic epidemiology topics.
Structured evidence summary
Research question
The study asked what added value routine whole genome sequencing provides for detecting drug resistance in Mycobacterium tuberculosis isolates in New South Wales, Australia.
Study design
This was a genomic study comparing resistance markers inferred from whole genome sequencing with commercial genotypic drug susceptibility tests and conventional phenotypic testing. The comparison used isolates sequenced from 2016 through 2019.
Population and setting
The study included 1107 clinical Mycobacterium tuberculosis isolates from New South Wales, Australia, sequenced between 2016 and 2019. The abstract describes the setting as low incidence.
Main findings
Among the sequenced isolates, 29 were multidrug resistant, and the most common lineages were Beijing and East-African Indian. Relative to phenotypic testing, whole genome sequencing indicated an additional 1% of isolates as likely resistant; relative to commercial genotypic assays, it increased resistance detection by 20%, with statistically significant gains that differed by lineage.
Public-health relevance
The abstract frames routine sequencing as relevant to tuberculosis drug resistance detection and management in low-incidence settings. The article is indexed to tuberculosis, antimicrobial resistance, and genomic epidemiology topics.
Important limitations
The abstract directly indicates that findings came from clinical isolates in one Australian jurisdiction and that detection gains varied across phylogenetic lineages. This summary is limited to the supplied single-article abstract and metadata; the full paper would be needed for decision-grade interpretation.
GIDS interpretation
For GIDS-style indexing, this article is discoverable in the context of tuberculosis, antimicrobial resistance, and genomic epidemiology, with bibliographic metadata identifying it as a peer-reviewed genomic study. The supplied classifier links do not list a country, although the abstract specifies New South Wales, Australia.
Related GIDS surveillance
Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.
Evidence relationships
This article has 6 auditable classifier relationships to diseases, places, topics, and study design.