Impact of antigen and carrier substitution on the molecular weight and immunogenicity of the Pfs230D1–CRM197 malaria transmission-blocking vaccine
Vaccine·
- DOI
- 10.1016/j.vaccine.2026.128913
- PMID
- 42456560
- PMCID
- —
- OpenAlex
- W7168257327
- Study type
- Journal article
- Publisher
- Elsevier BV
- Article type
- journal-article
- Integrity
- current
Why this research matters now
The findings provide preclinical formulation and manufacturing context for development of a Pfs230D1-CRM197 malaria transmission-blocking vaccine. The supplied evidence does not report human immunogenicity, transmission reduction, clinical effectiveness, or population-level impact.
Structured evidence summary
Research question
How do differing levels of sulfhydryl modification on Pfs230D1 and maleimide modification on CRM197 affect the molecular weight and immunogenicity of Pfs230D1-CRM197 conjugates?
Study design
The study generated a series of Pfs230D1-CRM197 conjugates with varied antigen and carrier modification levels, followed by a mouse immunogenicity study using Matrix-M adjuvant.
Population and setting
The reported immunogenicity evaluation was conducted in mice using Matrix-M-adjuvanted conjugates. No human population or clinical setting is described in the supplied abstract.
Main findings
Conjugate size and immunogenicity varied with the numbers of sulfhydryl and maleimide modifications applied to the antigen and carrier. A wide range of modification levels produced highly immunogenic Pfs230D1 conjugates in the mouse study.
Public-health relevance
The findings provide preclinical formulation and manufacturing context for development of a Pfs230D1-CRM197 malaria transmission-blocking vaccine. The supplied evidence does not report human immunogenicity, transmission reduction, clinical effectiveness, or population-level impact.
Important limitations
The supplied abstract describes a mouse immunogenicity study and does not provide human, clinical, or population-level outcomes. This summary is limited to the supplied single-article abstract and metadata; the original paper is required for decision-grade interpretation.
GIDS interpretation
This article is discoverable as malaria vaccination and transmission-dynamics literature because it concerns a preclinical transmission-blocking vaccine candidate. It provides research context only and does not establish or confirm a live surveillance signal.
Related GIDS surveillance
Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.
Evidence relationships
This article has 8 auditable classifier relationships to diseases, places, topics, and study design.