Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox — Chongqing Municipality, China, 2022
China CDC Weekly·
- DOI
- 10.46234/ccdcw2022.206
- PMID
- 36483191
- PMCID
- PMC9713572
- OpenAlex
- W4311027438
- Study type
- Journal article
- Publisher
- Chinese Center for Disease Control and Prevention
- Article type
- journal-article
- Integrity
- current
Why this research matters now
The abstract states that the isolated strain and the reported infectious-titration and neutralization methods provide resources and technical platforms for further research, including antiviral and vaccine development.
Structured evidence summary
Research question
The study examined whether monkeypox virus could be isolated and characterized from the first reported imported mainland-China case in September 2022.
Study design
This was a laboratory-based investigation of clinical specimens from a reported monkeypox case. Virus isolation and characterization used cell culture, quantitative PCR, cytopathic-effect assessment, immunofluorescence, electron microscopy, plaque assays, and a plaque-reduction neutralization test.
Population and setting
The investigation involved specimens from the first imported monkeypox case reported in mainland China, in Chongqing Municipality. Samples included skin blister fluid, oropharyngeal and nasopharyngeal swabs, and blood.
Main findings
The investigators isolated and identified an MPXV strain designated MPXV-B.1-China-C-Tan-CQ01. Viral replication was confirmed, characteristic cytopathic effects appeared after 48 hours, the titer exceeded 10^6 PFU/mL after three passages, and the patient's day-6 neutralization titer was 35.
Public-health relevance
The abstract states that the isolated strain and the reported infectious-titration and neutralization methods provide resources and technical platforms for further research, including antiviral and vaccine development.
Important limitations
The study describes laboratory characterization from a single reported case, so the supplied abstract does not establish findings for broader patient populations or transmission patterns. This summary is limited to the supplied single-article abstract and metadata; the original paper would be required for decision-grade interpretation.
GIDS interpretation
The article is discoverable in the supplied metadata as concerning monkeypox in China, with classifier links to transmission dynamics, travel medicine, and vaccination. These links provide bibliographic context only and do not confirm or characterize a live surveillance signal.
Related GIDS surveillance
Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.
Evidence relationships
This article has 10 auditable classifier relationships to diseases, places, topics, and study design.