A rapid detection of Nipah virus nucleocapsid antigen in inactivated viral cultures.
Emerging microbes & infections·
- DOI
- 10.1080/22221751.2026.2716499
- PMID
- 42565473
- PMCID
- —
- OpenAlex
- W7201868839
- Study type
- Journal article
- Publisher
- Publisher unavailable
- Article type
- journal-article
- Integrity
- current
Why this research matters now
The paper is relevant to outbreak preparedness because it describes a rapid antigen test intended for Nipah virus detection in settings where nucleic-acid testing may be difficult to use. The abstract frames the assay as potentially useful for point-of-care diagnosis in resource-limited contexts.
Structured evidence summary
Research question
The study asked whether a cellulose nanobead-enhanced lateral flow immunoassay could rapidly detect Nipah virus nucleocapsid antigen.
Study design
This is a laboratory assay-development and analytical validation study. The authors selected and paired antibodies, then tested the finalized lateral flow assay with recombinant antigen and cultured virus preparations.
Population and setting
The work was conducted in a non-clinical laboratory setting using recombinant Nipah nucleocapsid protein and γ-irradiated cultured Nipah virus. No human participants or patient setting are described in the abstract.
Main findings
The finalized assay returned results in about 15 minutes and showed an analytical detection limit of 1 ng/mL for recombinant Nipah nucleocapsid protein. The abstract reports no cross-reactivity with several listed viruses, with weak measles signal only at a very high antigen concentration, and detection of irradiated cultured Nipah virus down to the stated copy-number thresholds by reader and by eye.
Public-health relevance
The paper is relevant to outbreak preparedness because it describes a rapid antigen test intended for Nipah virus detection in settings where nucleic-acid testing may be difficult to use. The abstract frames the assay as potentially useful for point-of-care diagnosis in resource-limited contexts.
Important limitations
The abstract does not describe clinical validation in patient samples or field use. The reported performance is limited to recombinant antigen and irradiated cultured virus, so the summary is constrained to the supplied single-article abstract/metadata and the original paper is needed for decision-grade interpretation.
GIDS interpretation
This article is discoverable as a peer-reviewed diagnostics study on Nipah virus and related outbreak-preparedness context. It provides assay-development context only and does not establish a live surveillance signal.
Related GIDS surveillance
Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.
Evidence relationships
This article has 7 auditable classifier relationships to diseases, places, topics, and study design.