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Peer reviewedOpen accessTyphoid feverCOVID-19DengueMalariaTyphusScrub Typhus

Azithromycin with or without cefixime for suspected or culture-confirmed uncomplicated typhoid fever in Nepal, Bangladesh, and Pakistan (ACT-South Asia): a double-blind, parallel-group, randomised, placebo-controlled, phase 4 trial

The Lancet Infectious Diseases·

Buddha Basnyat, Sudeep Adhikari, Puja Shrestha, Saraswati Budhathoki, Md Shabab Hossain, Naveed Ahmed, Sabina Dongol, Nhukesh Maharjan, Olita Shilpakar, Damodar Gajurel, Dhruba Shrestha, Sanjib Kumar Sharma, Sadia Shakoor, Yuba Nidhi Basaula, Shiva Ojha, Lochan Karki, Evelyne Kestelyn, Marcel Wolbers, Guy E Thwaites, Tahmeed Ahmed, Farah Naz Qamar, Abhilasha Karkey, Christopher M Parry, Jyoti Acharya, Sharmila Acharya, Imran Ahmed, Sharmin Akter, Amit Arjyal, Milan Bajracharya, Stephen Baker, Sunil Baniya, Tran Nguyen Bao, Rohan Basnet, Adwoa Bentsi-Enchill, Dilli Bahadur Bhandari, Parmananda Bhandari, Pragya Bhandari, Pratik Bhattarai, Sonika Bhattarai, Urza Bhattarai, Renu Bhuju, Zulfiqar Bhutta, Durga Bishra, Sumit Bista, Bimal Kumar Bista, Probaha Biswas, Lalita Bokati, Bimal Kumar Chalise, Saheb Lal Choudhary, John Crump, Sadana Dahal, Ashata Dahal, Abul Faiz, Irum Fatima, Sumit Gami, Aastha Ghimire, Abhishek Giri, Rebecca Grais, Swornim Gyawali, Naheeda Haque, Caroline Harris, Ho Hien, Lan Le Thi Hoang, Md Mahbubul Hoque, Aneeta Hotwani, Mohsina Ibrahim, Shaumik Islam, Prachanda Japrel, Brajesh Kumar Jha, Rajesh Dhoj Joshi, Bimala Joshi, Juni Joshi, Manij Joshi, Subi Joshi, Laila Kabir, Md. Kamruzzaman, Bijaya Karanjit, Sushila Karki, Rinu Kayastha, Ashmita KC, Milan Khadka, Nisha Khadka, Ar-Rafi Khan, Basudha Khanal, Januka Khatri, Niraj Khatri, Sweta Koirala, Ranjit Kumar, Narayan Kunwar, Santosh Lamsal, Sue Lee, Sunil Limbu, Sangita Thapa Magar, Archana Maharjan, Shrawan Kumar Mandal, Suneeta Namdave, Nosheen Nasir, Bal Mukunda Neupane, Jenish Neupane, Nowrin Nusrat Nova, Shiva Krishna Pandey, Binod Panta, Nirmala Pariyar, Sushmita Pathak, Santosh Paudel, Shankar Paudel, Anupama Pokharel, Seema Poudyal, Buddhi Prasad Poudyal, Alisha Pradhan, Rajana Pyatha, Sonia Qureshi, Bhawana Rai, Mamit Rai, Manisha Rawal, Bijaya Rawat, Ajit Rayamajhi, Isabella Ribeiro, Samita Rijal, Santa Rokaya, Binita Rupakheti, G.K.M. Shahiduzzaman, Lubaba Shahrin, Ronas Shakya, Chandrama Sharma, Dibya Sharma, Karan Sharma, Upama Sharma, Bidhya Laxmi Shrestha, Basudha Shrestha, Divesh Shrestha, Medina Shrestha, Milan Shrestha, Raj Shrestha, Rajendra Shrestha, Sarita Shrestha, Shova Shrestha, Suchita Shrestha, Surendra Shrestha, Sushmita Shrestha, Keshab Raj Sigdel, Pawan Singh, Shraddha Subedi, Sapna Sunar, Anjana Tachamo, Afia Tariq, Devendra Singh Thagunna, Duy Thanh, Lich Bui Thanh, Jyoti Thapa, Rajkumar Thapa, Monika Thimi, Dikshya Sharma Timilsina, Thuan Dang Trong, Siddhartha Upadhaya, Nick White, Ram Babu Yadav, Ajay Kumar Yadav

DOI
10.1016/s1473-3099(26)00358-0
PMID
42624818
PMCID
OpenAlex
W7203820018
Study type
Journal article
Publisher
Elsevier BV
Article type
journal-article
Integrity
current

Why this research matters now

The findings support the WHO recommendation of oral azithromycin monotherapy for uncomplicated typhoid fever and provide no evidence to justify adding cefixime, which has important antimicrobial stewardship implications given concerns about dual-drug resistance emergence.

01

Structured evidence summary

Research question

The trial assessed whether combining azithromycin with cefixime reduces treatment failure in uncomplicated typhoid fever compared to azithromycin alone.

Study design

This was a double-blind, parallel-group, randomized, placebo-controlled phase 4 trial conducted across three South Asian countries. Participants were randomly assigned 1:1 to receive either azithromycin plus cefixime or azithromycin plus placebo for seven days, with treatment allocation concealed from all personnel and participants.

Population and setting

The study enrolled 1847 adults and children aged 2-65 years with blood culture-confirmed or clinically suspected uncomplicated typhoid fever from emergency and outpatient clinics in Nepal, Bangladesh, and Pakistan. Participants presented with acute undifferentiated febrile illness lasting 3-14 days and elevated C-reactive protein, with negative tests for dengue, scrub typhus, malaria, and COVID-19.

Main findings

Treatment failure occurred in 5.1% of participants in both the azithromycin-cefixime group and the azithromycin-placebo group, with no significant difference between arms. Among culture-confirmed cases, failure rates were 11.2% with combination therapy versus 16.0% with azithromycin alone, but this difference was not statistically significant. Adverse event rates were comparable between groups at 16% each.

Public-health relevance

The findings support the WHO recommendation of oral azithromycin monotherapy for uncomplicated typhoid fever and provide no evidence to justify adding cefixime, which has important antimicrobial stewardship implications given concerns about dual-drug resistance emergence.

Important limitations

This summary relies on the supplied single-article abstract and metadata. Full interpretation of trial limitations, including power calculations, adherence rates, and subgroup analyses, requires review of the complete published manuscript.

GIDS interpretation

The article is indexed under typhoid fever treatment in three endemic South Asian countries and is discoverable through disease and geographic classifiers. It addresses a clinical management question rather than documenting surveillance trends or outbreak characteristics.

02

Related GIDS surveillance

Literature context does not validate, explain, or change a surveillance signal. Exact and contextual relationships are shown separately.

03

Evidence relationships

This article has 8 auditable classifier relationships to diseases, places, topics, and study design.

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